| Week 1 |
Aug 23 |
Overview, Safety, Notebooks. Micropipettes and Buffers. |
powerpoint file, pdf file
protocol
|
| Week 2 |
Aug 30 |
Genomic DNA extraction |
powerpoint file,
pdf file
protocol,
(pdf)
|
| Week 3 |
Sep 6 |
A. Exam 1 on DNA extraction
B. Lecture on agricultural biotechnology
C. visit to Monsanto MTU |
powerpoint file
|
| Week 4 |
Sep 13 |
Extraction of RNA |
powerpoint file,
pdf file
protocol,
protocol in pdf |
| Week 5 |
Sep 20 |
A. Exam 2 on RNA extraction
B. RNA electrophoresis, photograph and blot
C. Discuss DNA gel blots |
powerpoint file, pdf file
protocol, (pdf) |
| Week 6 |
Sep 27 |
A. Detection of probe hybridization
B. Discuss probe production methods |
powerpoint file, pdf file
protocol, (pdf) |
| Week 7 |
Oct 4 |
A. Exam 3 on gel blots
B. PCR amplification of a DNA fragment from genomic soybean DNA
C. Separation of PCR products by gel electrophoresis |
powerpoint file, pdf file
protocol, (pdf) |
| Week 8 |
Oct 11 |
A. Exam 4 on PCR
B. Ligate PCR product into a plasmid vector
C. Transform ligation products into E. coli . |
powerpoint file, pdf file
protocol, (pdf) |
| Week 9 |
Oct 18 |
A. Exam 5 on ligation and transformation
B. PCR amplify the plasmid insert from bacterial colonies
C. Use agarose gel electrophoresis to determine DNA size |
powerpoint file, pdf file
protocol, (pdf) |
| Week 10 |
Oct 25 |
A. Discuss DNA sequencing
B. Computer analysis of DNA sequences
C. Homework Project (Exam 6) handed out to take home (double points!)
|
powerpoint file, pdf file
home assignment pdf format,
Gel results
Sequencing output (f), Sequencing output (r), control sequence. |
| Week 11 |
Nov 1 |
No class this week |
|
| Week 12 |
Nov 8 |
A. Exam 7 on DNA sequencing
B. Expression of a recombinant soybean protein in E. coli .
C. Analyze proteins using SDS - PAGE
D. Stain proteins in a SDS - PAGE gel
|
powerpoint file, pdf file
protocol, (pdf)
|
| Week 13 |
Nov 15 |
A. Hand in Homework (Exam 6) on bioinformatics
B. Exam 8 on protein analysis
C. Discuss the results of protein expression in E. coli
D. Purify the protein using affinity chromatography.
E. Run a new protein gel |
powerpoint file, pdf file protocol, (pdf)
|
| |
Nov 22 |
Thanksgiving break |
|
| Week 14 |
Nov 29 |
A. Exam 9 on protein purification
B. Electroblot proteins onto nitrocellulose (Demonstration)
C. Immunodetection of proteins (Western blot) |
powerpoint file, pdf file protocol, (pdf)
|
| Week 15 |
Dec 7 |
A. Functional Genomics and Proteomics
B. Review of laboratory notebooks
| powerpoint file, pdf file |
