| Week 1 |
Aug 27 |
Overview, Safety, Notebooks. Micropipettes and Buffers. |
powerpoint file, pdf file
protocol
|
| Week 2 |
Sep 3 |
Genomic DNA extraction |
powerpoint file,
pdf file
protocol,
(pdf)
|
| Week 3 |
Sep 10 |
No class |
|
| Week 4 |
Sep 17 |
A. Exam 1 on DNA extraction
Extraction of RNA. |
powerpoint file
pdf file
protocol,
protocol in pdf |
| Week 5 |
Sep 24 |
Exam 2 on RNA extraction. RNA electrophoresis, photograph and blot. Discuss DNA gel blots. |
powerpoint file,
pdf file
protocol,
protocol in pdf |
| Week 6 |
Oct 1 |
Detection of probe hybridization. B. Discuss probe production methods.
| powerpoint file, pdf file protocol, (pdf) |
| Week 7 |
Oct 8 |
Exam 3 on gel blots. PCR amplification of a DNA fragment from genomic soybean DNA. Separation of PCR products by gel
electrophoresis.
| powerpoint file, pdf file
protocol, (pdf) |
| Week 8 |
Oct 15 |
A. Exam 4 cancelled this week
B. Ligate PCR product into a plasmid vector
C. Transform ligation products into E. coli . |
powerpoint file, pdf file
protocol, (pdf) |
| Week 9 |
Oct 22 |
A. Exam "4+5" on PCR, ligation and transformation
B. PCR amplify the plasmid insert from bacterial colonies
C. Use agarose gel electrophoresis to determine DNA size |
powerpoint file, pdf file
protocol, (pdf) |
| Week 10 |
Oct 29 |
DNA Sequencing and High Throughput Technology |
powerpoint file, pdf file home assignment pdf format
colony picker robot movie,
biomek robot movie,
Roche movies,
Illumina movie,
Pac Biosci movie
Sequencing output 1 (f), Sequencing output 2 (f) Sequencing output 1 (r), Sequencing output 2 (r),
failed sequence output,
control sequence. |
| Week 11 |
Nov 5 |
A. Discuss DNA sequencing
B. Computer analysis of DNA sequences
C. Homework Project handed out to take home.
| powerpoint file, pdf file
|
| Week 12 |
Nov 12 |
A. Exam 6 on sequencing and analysis
B. Expression of a recombinant soybean protein in E. coli .
C. Analyze proteins using SDS - PAGE
D. Visit to functional genomics and proteomics while gel is running.
|
powerpoint file, pdf file protocol, (pdf)
|
Week 13 |
Nov 19 |
A. Exam 7 on protein expression
B. Hand in homework on Bioinformatics
C. Discuss the results of protein expression in E. coli
D. Purify the protein using affinity chromatography.
E. Run a new protein gel |
powerpoint file, pdf file protocol, (pdf)
|
| |
Nov 26 |
Thanksgiving break |
|
| Week 14 |
Dec 3 |
A. Exam 8 on protein purification
B. Electroblot proteins onto nitrocellulose (Demonstration)
C. Immunodetection of proteins (Western blot) |
powerpoint file, pdf file protocol, (pdf)
|
